Structural Analysis of 14-3-3 Phosphopeptide Complexes Identifies a Dual Role for the Nuclear Export Signal of 14-3-3 in Ligand Binding
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چکیده
منابع مشابه
The Structural Basis for 14-3-3:Phosphopeptide Binding Specificity
The 14-3-3 family of proteins mediates signal transduction by binding to phosphoserine-containing proteins. Using phosphoserine-oriented peptide libraries to probe all mammalian and yeast 14-3-3s, we identified two different binding motifs, RSXpSXP and RXY/FXpSXP, present in nearly all known 14-3-3 binding proteins. The crystal structure of 14-3-3zeta complexed with the phosphoserine motif in p...
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Forward transport of proteins from the ER to the plasma membrane requires escape from the ER's retention machinery. Recent studies suggest that 14-3-3 proteins may mediate ER export of potassium channels destined for the plasma membrane by interfering with dibasic-motif-mediated retention.
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There was an error published in J. Cell Sci. 119, 3695–3704. The GST-14-3-3 Coomassie stain shown in Fig. 2B was inadvertently duplicated from that shown in Fig. 2A. In Fig. 2C, the left-hand and middle GST Coomassie stains were also inadvertently duplicated, and annotation of the re-ordering of the GST-BD42 and GST-BD42A samples was omitted. Given that the data are more than 10 years old, the ...
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MOTIVATION The 14-3-3 family of phosphoprotein-binding proteins regulates many cellular processes by docking onto pairs of phosphorylated Ser and Thr residues in a constellation of intracellular targets. Therefore, there is a pressing need to develop new prediction methods that use an updated set of 14-3-3-binding motifs for the identification of new 14-3-3 targets and to prioritize the downstr...
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The 14-3-3 proteins are a highly conserved family of homodimeric and heterodimeric molecules, expressed in all eukaryotic cells. In human cells, this family consists of seven distinct but highly homologous 14-3-3 isoforms. 14-3-3σ is the only isoform directly linked to cancer in epithelial cells, which is regulated by major tumor suppressor genes. For each 14-3-3 isoform, we have 1,000 peptide ...
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ژورنال
عنوان ژورنال: Molecular Cell
سال: 1999
ISSN: 1097-2765
DOI: 10.1016/s1097-2765(00)80363-9